GALLIC ACID AND GLUTAMIC ACID CRUDE EXTRACT of SAN-SANGK (Albertisia papuana Becc.) LEAVES by Aspergillus niger FNCC 6018 FERMENTATION at pH and TEMPERATURE VARIETY CONDITION

Ani Nurhayati, Sulvi Purwayanti, Sholahudin .

Abstract


One of methods to gallic acid plant productionbytannin fermentation using Aspergillus microbes. The purposeof this research were to obtain gallic acid and glutamic acid from san-sangk (Albertisia papuana Becc.) leaves fermentation using Aspergillus niger FNCC 6018. Research design used factorial block randomized between temperature (25; 30; 45°C) and pH (5; 5.5; 6). The data such as total of tannin (TT) was measuring as a tannic acid while gallic acid was measured from total of phenolic (TF) and glutamic acidwas measured from total of free amino acid (TAAB). Data was analyzed byAnova (α= 5%).The result showed that there were not significantly between temperature and pH to TT, TF and TAAB.The treatmen with lower of total tannin 0.579 mg/100 g and higher of total phenolic 0.224 mg GAE/g is combination between temperature of 30oC and pH of 5.5 for 48 hours. The present work showed that tannin hydrolysis efficiency of Albertisia papuana using this method was 41.7%.


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