Uji Aktivitas Antibakteri Fraksi Metanol, Etil Asetat, dan N-Heksana Rimpang Lengkuas Merah (Alpinia purpurata, K. Schum) terhadap Bakteri Propionibacterium acnes

Abstract

Red galangal rhizome (Alpinia purpurata K. Schum) is a plant that is known to be used as a medicine for skin diseases. Acne is a skin disease that usually appears on the face caused by the overactive activity of the skin’s oil glands and is exacerbated by infection with the Propionibacterium acnes bacteria. This study aims to determine the antibacterial activity and the most active concentration of each red galangal rhizome fraction on the growth of P. acnes bacteria. This study used the disk diffusion method with three types of solvents namely methanol, ethyl acetate and n-hexane with various concentrations of 5%, 10%, 15%, and 20% and used tetracycline as a comparison and DMSO 10% as a control. Parameters observed were the diameter of the growth inhibition zone of P.acnes bacteria and were analyzed using ANOVA (Analysis of Variance). The results of the antibacterial activity test showed that the methanol, ethyl acetate, and n-hexane fractions of red galangal (A. purpurata) rhizome had antibacterial activity. The concentration of the most active fraction in inhibiting the growth of P. acnes bacteria was the methanol fraction with a concentration of 20% with an average inhibition zone diameter of 24.33 mm during the 24 hour incubation period and was bacteriostatic