IN VITRO BACTERIA CAPACITY EVALUATION OF Pseudomonas putida AND Staphylococcus aureus AS BIOREMEDIATION AGENT FOR LEAD HEAVY METAL
Abstract
Various biological such as bacteria used as alternative agent to decrease metal ion in waste water, one of which is Pb metal. The bacteria used in this study were P.putida and S.aureus. The sample in this research use Pb 1, 3, and 5 ppm solution. Bioremediation experiments performed with addition of suspension of each bacteria with concentrations of 5, 10, and 15% (v/v) and using incubator shaker with temperature 37˚C. The Pb metal ion content and bacterial growth analysis on 0, 1, 10, 20 and 30 days, using Automic Absorption Spectrophotometer (AAS) and Total Plate Count (TPC) method respectively. The results showed the decreasing percentage of Pb metal ion content in P.putida is the highest decreasing in Pb weight of 88%, with bacterial inoculum P.putida 15%(v/v) while in S.aureus sample was 99% with bacterial inoculum 15%(v/v). The ability of Gram-positive bacteria to reduce Pb-weight is more effective in bioremediation in aquatic waste than Gram-negative bacteria.
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