MORPHOLOGICAL AND CHROMOSOMAL CHARACTERIZATION OF ORCHID Peristylus goodyeroides Lindl. FROM CURUG SETAWING, KULONPROGO

Febri Yuda Kurniawan, Anindita Della Rosa Riyadi

Abstract


Peristylus goodyeroides is a terestrial orchid that scattered around Southeast Asia. Morphological characters of P. goodyeroides can vary, depending on the ecological factors and habitat. Cytological characters in the form of chromosome configurations can be used as a taxonomic tool for the process of identifying and understanding variations in taxa. The purpose of this study was to determine the morphological characters and chromosome configuration of the P. goodyeroides from Curug Setawing, Kulonprogo. The method used was morphological characterization and plant chromosome preparation by squash method with the main steps of fixation, maceration, staining and observation. Data were analyzed with the help of Image raster 3, Corel Draw X7, and Microsoft Excel 2013. P. goodyeroides from Curug Setawing has the morphological characters of root tubers, cylindrical stems, ovate leaf shape, convolutive leaf arrangement and creamy white flowers. The orchid has a number of chromosomes 2n = 10 with a karyotype formula of 2n = 2x = 8m + 2t. Metacentric chromosomes are found on chromosomes 1-8 and telocentric chromosomes 9-10. The absolute arm length of the chromosomes has a range of 2.03-3.44 μm, the relative arm length of the chromosomes is 2.21-3.32 μm, the length of the p arm is 1.13-1.58 μm and the q arm is 1.23-2.12 μm.


Keywords


chromosome; Curug Setawing; Peristylus goodyeroides; karyotype; morphology

Full Text:

PDF

References


Aristya, G.R., Daryono, B.S., Handayani, N. S.N., & Arisuryanti, T. (2015). Karakterisasi kromosom tumbuhan dan hewan. Gadjah Mada University Press. Yogyakarta.

Aristya, G.R., Zuyyina, C., Febiansi, D., Ayuningsih, R., Prasiwi, K.D., Nurwijayanti, T.A., Mujahidah, U. & Renaldy, B. (2019). Karakterisasi kromosom spesies anggota Familia Solanaceae. BIOTROPIC: The Journal of Tropical Biology 3(1): 24-38.

Ata, A.M., Abo-Bakr, M.A., El-Mazny, M.Y., Galal, A.A., Abd El-Twab, M.H., & Ramadan, A.M.M. (2017). A study of karyotype and mitotic chromosome behavior of some pea (Pisum sativum L.) genotypes cultivated in Eygpt. Minia Journal of Agriculture Research and Development 37(4): 587-603.

Bocianowski, J., Warzecha, T., Nowosad, K., & Bathelt, R. (2019). Genotype by environment interaction using AMMI model and estimation of additive and epistasis gene effects for 1000-kernel weight in spring barley (Hordeum vulgare L.). Journal of Applied Genetics 60(1): 127–135.

Bolcun-Filas, E. & Schimenti, J.C. (2012). Genetics of Meiosis and Recombination in Mice. International Review of Cell and Molecular Biology 298(1): 179-227.

Bzdega, K., Janiak, A., Książczyk, T., Lewandowska, A., Gancarek, M., Sliwinska, E., & Tokarska-Guzik, B. (2016). A survey of genetic variation and genome evolution within the invasive Fallopia complex. PLOS ONE 11(8): 1-23.

CCBD. (2021). The Chromosome Counts Database (CCDB) version 1.58. Retrieved April 03, 2021, from http://ccdb.tau.ac.il/

Christenhusz, M.J.M. & Byng, J.W. (2016). The number of known plants species in the world and its annual increase. Phytotaxa 261(3): 201–217.

Comber, J.B. 1990. Orchid of Java. The Royal Botanic Garden Kew. London.

Daviña, J.R., Grabiele, M., Cerutti, J.C., Hojsgaard, D.H., Almada, R.D., Insaurralde, I.S., & Honfi, A.I. (2009). Chromosome studies in Orchidaceae from Argentina. Journal of Genetics and Molecular Biology 32(4): 811–821.

Djordjević, V., Tsiftsis, S., Lakušić, D., Jovanović, S., & Stevanović, V. (2016). Factors affecting the distribution and abundance of orchids in grasslands and herbaceous wetlands. Systematics and Biodiversity 14(4): 355-370.

Erzurumlu, G.S., Sultana, N., Vural, M., & Serçe, S. (2018). Genetic and phenotypic variation among Turkish terrestrial orchid species as revealed by RAPD and morphological characteristics. Turkish Journal of Agriculture and Forestry 42(4): 227-236.

Felix, L., & Guerra, M. (2005). Basic chromosome numbers of terrestrial orchids. Plant Systematics and Evolution 254(1): 131–148.

Gienapp, P., Laine, V.N., Mateman, A.C., van Oers, K., & Visser, M.E. (2017). Environment-dependent genotype-phenotype associations in avian breeding time. Frontiers in Genetics 8(102): 1-9.

Google Earth. (2020). Maps of Curug Setawing location, Kulonprogo, Indonesia. Retrieved December 23, 2020, from https://earth.google.com/web/.

Hartati, S., Darsana, L., & Cahyono, O. (2014). Studi karakterisasi anggrek secara sitologi dalam rangka pelestarian plasma nutfah. Jurnal Ilmu-Ilmu Pertanian XXIX (1): 25-30.

Hartati, S., Nandariyah, Yunus, A., & Djoar, D.W. (2017). Short communication: cytological studies on black orchid hybrid (Coelogyne pandurata Lindley). Biodiversitas 18(2): 555-559.

Jalal, J.S. & Jayanthi, J. (2015). An annotated checklist of the orchids of western Himalaya, India. Lankesteriana 15(1): 7-50.

Jin, W.T., Schuiteman, A., Chase, M.W., Li, J.W., Chung, S.W., Hsu, T.C., & Jin, X.H. (2017). Phylogenetics of subtribe Orchidinae s.l. (Orchidaceae; Orchidoideae) based on seven markers (plastid matK, psaB, rbcL, trnL-F, trnH- psba, and nuclear nrITS, Xdh): implications for generic delimitation. BMC Plant Biology 17(222): 1-14.

Kurniawan, F.Y., Setiaji, A., Putri, F., Suyoko, A., & Semiarti, E. (2018). Diversity and conservation strategy of orchids under anthropogenic influence in Taman Wisata Alam Curug Setawing, Yogyakarta. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia 4(2):173-177.

Levan, A., Fredga, K. & Sandberg, A.A. (1964). Nomenclature for centromic potition on chromosomes. Institute of Genetics, Lund. Sweeden.

Lukhtanov, V.A., & Shapoval, N.A. (2017). Chromosomal identification of cryptic species sharing their DNA barcodes: Polyommatus (Agrodiaetus) antidolus and P. (A.) morgani in Iran (Lepidoptera, Lycaenidae). Comparative cytogenetics 11(4): 759–768.

Luo, Y.B. (2004). Cytological studies on some representative species of the Tribe Orchideae (Orchidaceae) from China. Botanical Journal of the Linnean Society 145(2): 231-238.

Lusinska, J., Betekhtin, A., Lopez-Alvarez, D., Catalan, P., Jenkins, G., Wolny, E., & Hasterok, R. (2019). Comparatively barcoded chromosomes of Brachypodium perennials tell the story of their karyotype structure and evolution. International journal of molecular sciences 20(22): 1-19.

Mártonfiová, L. (2013). A method of standardization of chromosome length measurement. Caryologia 66(4): 304-312.

Nagara, R.P. & Buchori, I. (2013). Pembangunan model keruangan untuk identifikasi kawasan rawan erosi di wilayah pesisir (studi kasus: Kabupaten Kulonprogo). Jurnal Teknik PWK, 2(4): 948-957.

Nurfadilah, S. (2017). Population study of Peristylus goodyeroides (Orchidaceae) in five habitats and implication for its conservation. Biodiversitas 18(3): 1084-1091.

Nurfadilah, S. (2017). Utilization of orchids of Wallacea region and implication for conservation. IOP Conference Series: Earth and Environmental Science 473(012063): 1-13.

Pamarthi, R.K., Devadas, R., Raj-kumar, Rai, D., Kiran-Babu, P., Meitei, A.L., De, L.C., Chakrabarthy, S., Barman, D., & Singh, D. R. (2019). PGR diversity and economic utilization of orchids. International Journal of Current Microbiology and Applied Sciences 8(10): 1865-1887.

Raskoti, B.B., Schuiteman, A., Jin, W.T., & Jin, X.H. (2017). A taxonomic revision of Herminium L. (Orchidoideae, Orchidaceae). PhytoKeys 79(1): 1–74.

Rice, A., Glick, L., Abadi, S., Einhorn, M., Kopelman, N.M., Salman-Minkov, A., Mayzel, J., Chay, O., & Mayrose, I. (2015) The Chromosome Counts Database (CCDB) - a community resource of plant chromosome numbers. New Phytologist 206(1):19-26.

Samatadze, T.E., Yurkevich, O.Y., Hazieva, F.M., Konyaeva, E.A., Morozov, A.I., Zoshchuk, S.A., Amosova, A.V., & Muravenko, O.V. (2020). Agro-morphological, microanatomical and molecular cytogenetic characterization of the medicinal plant Chelidonium majus L. Plants (Basel, Switzerland) 9(10): 1-17.

Setiaji, A., Muna, A., Jati, F.P., Putri, F., & Semiarti, E. (2018). Keanekaragaman anggrek di Daerah Istimewa Yogyakarta. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia 4(1): 63-68.

Sharma, S.K. & Mukai, Y. (2015). Chromosome research in orchids: current status and future prospects with special emphasis from molecular and epigenetic perspective. Nucleus 58(3): 173–184.

Shetty, N.K. (2018). Inheritance of chromosomes, sex determination, and the human genome: a new paradigm. Gender and the Genome 2(1): 16–26.

Singh, R.J. (2003). Plant Cytogenetics. CRC Press. New York.

Suliartini, N., Purwantoro, A., & Sulistyaningsih, E. (2004). Keragaman genetik dalam spesies Caladium bicolor berdasarkan analisis kariotipe. Agrosains 17(2): 235-244.

Wang, J.H. & Zheng, X.D. (2017). Comparison of the genetic relationship between nine Cephalopod species based on cluster analysis of karyotype evolutionary distance. Comparative cytogenetic 11(3): 477–494.

Young, H.A., Sarath, G. & Tobias, C.M. (2012). Karyotype variation is indicative of subgenomic and ecotypic differentiation in switchgrass. BMC Plant Biology 12(117): 1-12.

Zhang, S., Yang, Y., Li, J., Qin, J., Zhang, W., Huang, W., Hu, H. (2018). Physiological diversity of orchids. Plant Diversity 40(4): 196-208.




DOI: http://dx.doi.org/10.26418/jpmipa.v12i2.41811

Refbacks

  • There are currently no refbacks.


Abstracted/Indexed by:

S3    


PUBLISHED BY:

Fakultas Keguruan dan Ilmu Pendidikan Universitas Tanjungpura
IN COOPERATION WITH
Perkumpulan Pendidikan IPA Indonesia (PPII)

Creative Commons License
Jurnal Pendidikan Matematika dan IPA is licensed under a Creative Commons Attribution 4.0 International License

View My Stats