Herlinda Djohan, Ari Widiyantoro, Anis Shofiyani


Flavonoid is one of the phenolic compounds that can complex metals. One of the native plants of Indonesia which has the potential as a metal complexor is the andong plant (Cordyline fruticosa (L.) A.Chev). The isolation process to obtain pure compound are extraction, fractionation, and separation by chromatography. Chromatography method using vacuum liquid chromatography (VLC), gravity column chromatography (GCC) and preparative thin layer chromatography (TLC). This research obtained relatively pure isolate F2.8. The IR (KBr) data on wave numbers of isolate F2.8 were 3430; 2925-2855; 1749; 1609-1512; 1171 and 1100 cm-1.The result of the spectrum 1H-NMR showed flavonoid compound has similar typical spectrum of apigenin compound which showed chemical shift (δH ppm) : 6.85 (1H, s, H-3); 6.29 (1H, s H-6); 6.57 (1H, H-8); 7.03 (2H, d, J = 11.96 Hz, H-3’, H-5’); 8.03 (2H, d, J = 11.72 Hz, H-2’, H-6’). The UV-Vis (CH3OH) spectrum data of isolate F2.8 after the addition of Fe2+ showed a shift at λmax towards a greater direction with a batochromic shift which was caused a decrease in absorbance after the addition of iron (Fe2+) 20 ppm namely: 290 nm; 305 nm with respectively absorbances of 1.62 A; 0.42 A. Based on these data, isolate F2.8 is apigenin which is capable of complexing Fe2+.

Full Text:



. Rudolph, A. M., Hoffmand, J. I. E., and Rudolph, C. D., 2007, Buku Ajar Pediatri, EGC, Jakarta

. Safitri, R., Ernawaty, J., and Karim, D., 2015, Hubungan Kepatuhan Tranfusi dan Konsumsi Kelasi Besi Terhadap Pertumbuhan Anak dengan Thalasemia, JOM, 2:1475

. Nadia, 2010, Studi Penggunaan Obat pada Pasien Thalassemia Anak, Universitas Airlangga

. Ebrahimzadeh, M.A., Nabavi, S.M., and Nabavi, S.F., 2009, Correlation Between the in vitro Iron Chelating Activity and Poly Phenol and Flavonoid Contents of Some Medicinal Plants, Pakistan Journal of Biological Sciences, 12 (12): 934

. Fouedjou, R.T., Nguelefack-Mbuyo, E.P., Ponou, B.K., Nguelefack, T.B., Barboni, L. and Tapondjou, L.A., 2016, Antioxidant Activities and Chemical Constituents of Extracts from Cordyline fruticosa (L.) A. Chev. (Agavaceae) and Eriobotrya japonica (Thunb) Lindl, (Rosaceae), Pharmacologia,7 (2-3).

. Budi, I.N.S., Widiyantoro, A., and Nurlina, 2017, Senyawa Flavonoid dari Fraksi Etil Asetat Batang Tanaman Andong (Cordyline furticosa) dan Aktivitas Sitotoksiknya Terhadap Sel HeLa, Jurnal Kimia Khatulistiwa, 6 (4) : 56-59

. Cannell, R.J.P, 1998, Method In Biotechnology : Natural Products Isolation, Humana Pres Inc, Totowa New Jersey

. Supratman, U., 2010, Elusidasi Struktur Senyawa Organik, Widya Padjajaran, Bandung.

. Farianti, 2000, Kriteria Pembentukan Struktur Senyawa Kompleks Logam, Institut Teknologi Bandung, Bandung.

. Leopoldini, M., Russo, N., Toscana, M., 2011, The Molecular Basis of Working Mechanism of Natural Polyphenolic Antioxidants. Food Chemistry 125:288-306

. Niciforovic, N., Mihailovic, V., Maskovic, P., Solujic, S., Stojkovic, A., and Muratspahic, D.P., 2010, Antioxidant Activity of Selected Plant Species Potential New Sources of Natural Antioxidants, Food and Chemical Toxicology, 48:3125-3130

. Carey, F., and Sundberg, RJ., 2007, Advanced Organic Chemistry Part A: Structure and Mechanisms. Fifth edition. New York : Springer Science Business Media, LLC

. Manivannan, R., and Aeganathan, R., and Prabakaran, K., 2015., Antimicrobial and Antiinflamatory Flavonoid Constituents from The Leaves of Lawsonia inermis, The Journal of Phytopharmacology, 4 (4) : 212-216

. Wang, Y., Huang, Y., Xu, Z., and Wen, X., 2018, Extraction, Purification and Hydrolisis Behavior Apigenin-7-O-Glucoside from Chrysanthemum morifolim Tea, Molecules, 23 (11) : 29-33

. Rahate, K., and Rajasekaran, A., 2018, Isolation and Identification of Flavone Aglycone in Roots Desmostachya bipinnata Stapf, Indian Journal of Pharmaceutical Sciences, 80 (3).



  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Indonesian Journal of Pure and Applied Chemistry (IJoPAC)

p-ISSN : 2620-8571, e-ISSN : 2620-858X5

Office Address:

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Tanjungpura, Jl. Prof. Dr. Hadari Nawawi, Pontianak, Indonesia 78124

Contact Person: +6289644694946, +6281236728813

Email: [email protected]

Supported by: