Rini Muharini, Masriani Masriani, Rudiyansyah Rudiyansyah


Zamioculcas zamiifolia is an ornamental plant that had been used pharmacologycally as a traditional medicine plant. The aims of this research were to investigate the phytochemistry of n-hexane, chloroform and methanol extracts from the roots of Z. zamiifolia and to evaluate their biological activities as antioxidant and cytotoxic. All extracts were screened phytochemically using specific reagents and TLC technique to reveal the major component of each extract. The chloroform was obtained as the highest quantity of extract which gave positive test for steroids and flavonoids. All extracts were tested for their antioxidant activity using DPPH method and for their cytotoxic activity against two types of human cancer cell lines, HepG2 and T47D, and normal African green monkey kidney ephitelial cell lines, Vero. The methanol and chloroform extract exhibited antioxidant activity with  AA50 values of 180.0 and 431.5 µg/mL, respectively. Chloroform and methanol extracts showed potencial and moderate cytotoxicity towards T47D cell line with IC50 433.1 and 461.1µg/mL, respectively. None of the extracts was toxic against HepG2 and Vero cell lines.

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Frausin G, Lima RBS, Hidalgo AF, Ming LC, Pohlit AM. (2015). Plants of the Araceae family for malaria and related diseases: a review. Revista Brasileira de Plantas Medicinais, 17 (4), 657 – 666.

Arditti J, Rodriguez E. (1982). Dieffenbachia: uses, abuses and toxic constituents: a review. Journal of Etnopharmacology, 5 (3), 293 – 302

Khaksar G, Treesubsuntorn C, Thiravetyan P. (2017). Effect of exogenous methyl jasmonate on airborne benzene removal by Zamioculcas zamiifolia: The role of cytochrome P450 expression, salicylic acid, IAA, ROS and antioxidant activity. Enviromental and Experimental Botany, 138, 130 – 138.

Sriprapat W, Boraphech P, Thiravetyan P. (2014). Factor affecting xylene-contaminated air removal by the ornamental plant Zamioculcas zamiifolia. Enviromental Science and Pollution Research International, 21 (4), 2603 – 2610.

Le Moullec A, Juvik O.J, Fossen T. (2015). First identification of natural products from the african medicinal plant Zamioculcas zamiifolia. Fitoterapia, 106: 280 – 285.

Sarker SD, Latif Z, Gray AI. (2006). Methods in Biotechnology, Natural Products Isolation. Edisi 2. New Jersey. USA: Humana Press Inc.

Musa KH, Abdullah A, Kuswandi B, Hidayat MA. (2013). A novel high throughput method based on DPPH dry reagent array for determination of antioxidant activity. Food Chemistry, 141, 4102 – 4106.

McLaughlin JL, Rogers LL. (1998). The use of biological assays to evaluate botanicals. Drug Information Journal, 32, 513 – 524.

Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunology Methods, 65: 55-63

Prayong P, Barusrux S, Weerapreeyakul N. (2008). Cytotoxicity activity screening of some indigenous Thai Plants. Fitoterapia, 79, 598 – 601.

Waghray A, Murali AR, Menon KVN. (2015). Hepatocellular carcinoma: From diagnosis to treatment. World Journal of Hepatology, 7 (8), 1020 – 1029.

Masriani, Mustofa, Jumina, Sunarti, Enawaty E. (2014). Cytotoxic and pro-apoptic activities of crude alkaloid from sengkubak (Pynarrhena cauliflora (Miers) Diels) in human breast cancer T47D cell line. Scholars Academic Journal of Biosciences, 2 (5), 336 – 340.



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