DETEKSI KEBERADAAN BAKTERI RESISTEN LOGAM MERKURI (Hg) PADA PENAMBANGAN EMAS TANPA IZIN (PETI) DI SIMPI, SEKADAU, KALIMANTAN BARAT

Abdullah Abdullah

Abstract


The purpose of this research is to know the existence of mercury metal degradation bacteria in the activity of illegal gold mining (PETI) in Simpi Village, Belitang Hilir sub-district, Sekadau District, West Kalimantan. The samples were taken from one location with three different sampling points. Isolation of bacteria by pour plate method  in NA-HgCl medium. Detection of bacteria by paper disc method based on inhibit zone resistant of  Mueller-Hinton Agar (MHA) with mercury levels such as 10 mg/L, 20 mg/L and 30 mg/L . The results showed that it was found that one pure isolate (PP) showed the most resistant isolate to the mercury stress of 10 mg/L can be detected

Full Text:

PDF

References


. Fatimawali., Badaruddin, F., Yusuf, I. (2011). Isolasi Dan Identifikasi Bakteri Resisten Merkuri Dari Muara Sungai Sario Yang Dapat Digunakan Untuk Detoksifikasi Limbah Merkuri, Jurnal Ilmiah Sains, 11, 283.

. Rasmussen, L. D. C., Zawadsky, S. J., Binnerup, G. O. S. J., Sorensen., Kroer, N. (2008). Cultivation of Hard-To-Culture Subsurface Mercury-Resistant Bacteria and Discovery of New merA Gene Sequences, Department of Environmental Chemistry and Microbiology, National Environmental Research Institute, University of Aarhus, Frederiksborgvej 399, 4000 Roskilde, Denmark, Institute of Biology, University of Copenhagen, Solvgade 83H, 1307 Copenhagen K, Denmark. App. Environ. Microbiol, 12, 3795-3803.

. Gadd, G. M. (1992). Metals and Microorganisms: A Problem of Definition. FEMS Microbiol Lett, 100, 197-204.

. Priyadarshini, S. (2011). Isolation, Identification and biochemical analysis of Mercury Resistant Bacteria (MRB) from the effluent water of Rourkela Steel Plant, Orissa. Department of Life Science

. Nakamura, K. M., Sakamoto, F., Uchiyama., Yagi, O. (1990). Organomercurial Volating Bacteria in the Mercury-Polluted Sediment of Minamata Bay, Japan. Appl. Environ Microbiol, 56, 304-311.

. Shovitri, M., Zulaika, E., Koentjoro, M. P. (2010). Bakteri Tahan Merkuri dari Kali Mas Surabaya Berpotensi sebagai Agen Bioremediasi Merkuri. Jurnal Berkala Penelitian Hayati Ed. Khusus 4F. 1-6.

. Ulfa, A., Suarsini, E., Muhdhar, M. H. I. (2016). Isolasi dan Uji Sensitivitas Merkuri pada Bakteri dari Limbah Penambangan Emas di Sekotong B, arat Kabupaten Lombok Barat: Penelitian Pendahuluan, Proceeding Biology Education Conference, 3, 398-399.

. Smit, E., Wolters, A., Elsa, J. D. V. (1998). Self-Transmissible Mercury, Resistance Plasmid with Gene Mobilizing Capacity in Soil Bacterial Population, Influence of Wheat Roots and Mercury Addition, Appl. Environ. Microbiol, 64, 1210-1219.

. Pemerintah Republik Indonesia. Peraturan Pemerintah Nomor 82 tahun 2001 Tentang Pengelolaan Kualitas Air dan Pengendalian Pencemaran Air, Jakarta. 2001

. Nithya, C., Gnanalakshmi, B,. Pandian, S. S. (2011). Assessment and characterization of Heavy Metal Resistant in Palk Bay Sediment Bacteria, Marine Environmental Research, 71, 283-294.

Silver, S. (1996). Bacterial Resistances to Toxic Metal Ion –a review 1, gene, 179, 9-19.




DOI: http://dx.doi.org/10.26418/indonesian.v1i2.30529

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

------------------------------------------------------------------------------

Indonesian Journal of Pure and Applied Chemistry (IJoPAC)

p-ISSN : 2620-8571, e-ISSN : 2620-858X5

Office Address:

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Tanjungpura, Jl. Prof. Dr. Hadari Nawawi, Pontianak, Indonesia 78124

Contact Person: +6289644694946, +6281236728813

Email: [email protected]

Supported by: