KARAKTERISTIK BAKTERI Xanthomonas (SL3) HASIL ISOLASI DARI DAUN PADI IR 64 BERGEJALA HAWAR DI KABUPATEN KUBU RAYA

Suprih Larasaty, Mukarlina Mukarlina

Abstract


Rice is a staple crop consumed by almost all Indonesians. One of the important diseases of rice plants is leaf blight, which can damage rice from the seedling phase to harvest. This study aims to determine the morphological and physiological characteristics of the bacterial genus isolated from the leaves of the rice plant IR 64 showing blight symptoms in Kubu Raya Regency, West Kalimantan. This research was conducted from May 2019 – September 2019 at the Biology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak. Sampling was conducted by inspecting the leaves of rice plants for symptoms of blight in the rice production center of Kubu Raya Regency. Identification is done by characterizing the bacteria morphologically and physiologically using the identification guide Bergey's Manual of Systematic Bacteriology. The bacterial genus isolated from IR 64 rice leaves with blight symptoms in Kubu Raya Regency is Xanthomonas, with the isolate code SL3.


References


Agarwal, VK, & Sinclair, JB, 1987. Seedborne Pathogens, In Principles of Seed Pathology, CRC Press, Inc, Florida Vol. 1, hal. 17-76.

BPS. 2016. Statistik Pertanian Tanaman Pangan (Padi dan Palawija) Kabupaten Kubu Raya, Badan Pusat Statistik, Kubu Raya, di akses 3 november 2018,

Bradbury, JF. 1986. Xanthomonas Dowson in: Guide to Plant Pathogenic Bacteria, CAB International Mycological Institute, Slough.

Fitriah, B, Irwan, L & Johanes, P. 2014. Eksplorasi terhadap Bakteri Endofit sebagai Agen Pengendali Hayati terhadap Penyakir Darah pada tanaman Pisang secara In Vitro, Jurnal Agrotekbis, 2(6): 579-578.

Hadioetomo, RS. 1993. Mikrobiologi Dasar dalam Praktik Teknik dan Prosedur Dasar Laboratorium, Gramedia, Jakarta

Hemraj, V, Diksha, S, & Avneet, G, 2013. A Review on Commonly Used Biochemical Test for Bacteria, Innovare Jurnal of Life Science, 1: 1-7

Herawati, A. 2016. Isolasi dan karakterisasi penyebab penyakit hawar daun bakteri (Xanthomonas oryzae pv. oryzae L.) pada tanaman padi di wilayah sulawesi selatan, Skripsi, Sekolah Tinggi Ilmu Pertanian YAPIM Maros, Sulawesi Selatan

Holt, JG, Krieg, NR, Sneath, PA, Staley, JT & Williams, ST. 1994. Bergey’s Manual of Determinative Bacteriology and ed, Williams and Wilkins, Baltimor

Hugh, R, & Leifson E. 1953. the Taxonomic Significance of Fermentative Verses Oxidative Metabolism of Carbohydrates by Various Gram Negative Bacteria, Journal Bacteriol, 66: 24- 26

David, O, Nino-Liu, Pamela, C, Ronaldand Adam, J, Bogdanove. 2006. Xanthomonas oryzae Pathovars, Model Patogen of a Model Crop, Mol Plant Path, 7: 303-324

2006.00344.x.>

Meynell, GG & Meynell, E. 1970. Theory and Practice in Experimental Bacteriology, 2nd Edition, Cambridge University Press, London.

Sands, D, S, 1990, Physiological Criteria- Determinative Test, In Klement, Z, Rudolph, K, and Sands, D, C, Methods in

Phytobacteriology, Akademisi Kado, Budapes

Semangun, H. 2000. Penyakit-Penyakit Tanaman Perkebunan di Indonesia, Gadjah Mada University, Yogyakarta.

Sudarsono, A. 2008. Isolasi dan Karakterisasi Bakteri pada Ikan Laut dalam Spesies Ikan Gindara (Lepidocibium flavobronneum), Skripsi. Institut Pertanian Bogor, Bogor.

Sudir, TS, Kadir, & Suprihanto, 2009, Identifikasi Patotipe Xanthmonas oryzae pv. oryzae, Penyebab Penyakit Hawar Daun Bakteri Padi di Sentra Produksi Padi di Jawa. Jurnal Penelitian Pertanian Tanaman Pangan, 28(3): 131-138.

Suparyono, Sudir, B, & Suprihanto. 2004. ‘Pathotype Profile of Xanthomonas Campestris pv. Oryzae, Isolates from The Rice Ecosystem in Java’ Indonesian Journal of Agricultural Science, 5(2): 63-

Syair, Samirin, Wijayanto, T, & Khaeruni, K. 2012. Pengelompokan Patotipe Xanthomonas oryzae pv. oryzae Asal Sulawesi Tenggara Menggunakan Padi Galur Isogenik IRRI. Jurnal Agroteknologi, 2(1): 41-49.

Tasliah, Mahrup, & Prasetiyono, J. 2013. Identifikasi Molekuler Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) dan Uji Patogenisitasnya pada Galur-galur Padi Isogenik, Jurnal AgroBiogen, 9(2): 49-57.

Triny, S, Kadir, Suryadi, Y, Sudir & Machmud,

M. 1998. Laporan Analisis Struktur Populasi Xanthomonas oryzae pv. oryzae untuk Menemukan Strategi Pengendalian, Balai Besar Penelitian Tanaman Padi, Subang

Winarni I. 2013. Isolasi dan Karakterisasi Bakteri Patogen pada Benih Padi dan Kedelai, Jurusan Biologi FMIPA, Universitas Terbuka, Jurnal Matematika, Sains, dan Teknologi, 14(2): 135-141.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

                                 













Jurnal BioEkuator (p-ISSN:; e-ISSN:)

Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak 78241, Indonesia

Phone: 082258303732; E-mail: bioekuator@untan.ac.id; URL: http://jurnal.untan.ac.id/index.php/bioekuator