Nurlina Nurlina, Intan Syahbanu, Mirna Tersiana Tamnasi, Chyntia Nabela, Maria Desi Furnata


Cow manure fertilizer was used as raw material for the extraction of humic acid in this research. The aim of this research were  extraction of humic acid from cow manure fertilizer, characterization of functional groups based on FTIR spectra, and determination of humic acid functional groups. Humic acid extraction from cow manure used 0.050 M; 0.100 M; 0.250 M dan 0.500 M KOH solution. A cow manure fertilizer were shaken with KOH solution for 1; 2; 4 and 6 h. The quantitative determination of oxygen-containing functional groups of humic acid was conducted by titrimetri. The FTIR spectrum showed the presence of  –OH group and N-H stretching (bands 3361.46 cm-1); C-H aliphatic for CH2, CH3 (2946.52 cm-1 and 1442.24 cm-1); C=C aromatic components (1626.48 cm-1); COO- symmetric stretching (1521.71 cm-1); C-O stretching from –COOH (1229.74 cm-1). Total acidity value was 500 cmole.kg-1 (Baryta Absorption Method); carboxyl groups content was 250 cmole.kg-1 (Ca-Acetate Method); and phenol (OH) concentration was 250 cmole.kg-1. It can be concluded that humic acid successfully extracted from cow manure fertilizer using 0.250 KOH solution as extractant for 2 h, which has  yield of 4.486% humic acid.

Full Text:



Wershaw R. L. Evaluation of Conceptual Models of Natural Organic Matter (Humus) From a Consideration of the Chemical and Biochemical Processes of Humification. Scientific Investigations Report 2004-5121U.S. Department of the Interior and U.S. Geological Survey Denver; 2004.

Bashir, M., Qadri, R. W. K., Khan, I., Zain, M., Rasool, A., & Ashraf, U. Humic acid application improves the growth, floret and bulb indices of Gladiolus (Gladiolus grandiflorus L.). Pakistan Journal of Science. 2016; 68 (2):121-127.

Xue, X., Xue, Z., Song, Z., Zhang, F., Wang, Z., & Han, Y. Effect of combined application of humic acid and urea on the wheat growth and yield. Asian Agricultural Research. 2016; 8 (9):67-70.

Wang, X., Su, Y., Xu, X., & Li, G. Effect of fulvic acid on growth and yield components of direct seeding rice, Agricultural Science & Technology. 2013; 14 (7):966-972.

Dai, M., Chen, Q., Hu, Z., Li, X., Zhang, J., Wang, J., & Feng, H. Effects of humic acid and PASP compound fertilizer application on growth, quality and yield of Broccoli, Agricultural Science & Technology. 2016; 17 (3):535-538.

Zandonadi D. B., Santos M. P., Busato J. G., Peres L.E.P, Façanha, A.R. Plant physiology as affected by humified organic matter, Theor. Exp. Plant Physiol. 2013; 25 (1):12-25.

Canellas LP,Spaccini R, Piccolo A, Dobbss LB, Façanha ALO, Santos GA, Olivares FL, and Façanha A.R. Relationships Between Chemical Characteristics and Root Growth Promotion of Humic Acids Isolated From Brazilian Oxisols, Soil Science. 2009; 174 (11):611-620.

Wijaya H. and Suwardi. Peningkatan Produksi Tanaman Pangan dengan Bahan Aktif Asam Humat dengan Zeolit sebagai Pembawa, Jurnal Ilmu Pertanian Indonesia. 2013; 18 (2):79.

Khaled H., and Fawy H.A. 2011. Effect of Different Levels of Humic Acids on the Nutrient Content, Plant Growth, and Soil Properties under Conditions of Salinity, Soil & Water Res. 2011; 6 (1):21-29.

Barot, N.S. and Bagla, H.K. Extraction of humic acid from biological matrix-dry cow dung powder. Journal Green Chemistry Letters and Review. 2009; 2 (4):217-221.

Nagasawa, K., Wang, B., Nishiya, K., Ushijima, K., Zhu, Q., Fukushima, M., Ichijo, T. Effects of humic acids derived from lignite and cattle manure on antioxidant enzymatic activities of barley root, J Environ Sci Health B. 2016; 51 (2):81-89.

Stevenson, F.J. 1994. Humus Chemistry: Genesis, Composition, Reaction. 2nd ed. John Wiley & Sons, Inc. New York.

Monedero, M.A.S., Cegarra, J., Garcia, D. and Roig, A. Chemical and structural evolution of humic acids during organic waste composting, Biodegradation. 2002; 13:361–371.

Fong, S.S., Lau S., Wong N.C., Janice A., Muhammad F., and Amira S, 2006, Characterization of the Coal Derived Humic Acids from Mukah Sarawak as Soil Conditioner, J. Braz Chem Soc. 2006; 17(2):582-587.

Bellera, C., Abaalkheel, I., Rovira, P. and Alrefai, J. Obtaining commercial humic products from uncomposted manures: previous acid hydrolysis to enhance yields, Int J Recycl Org Waste Agricult. 2015; 4:219–231.

Asing J , N.C. Wong and S. Lau. Optimization Of Extraction Method And Characterization Of Humic Acid Derived From Coals and Composts. J. Trop. Agric. and Fd. Sc. 2009; 37 (2): 211–223.

Astuti, P., Idiawati, N., dan Destiarti, L., 2015. Validasi Metode Pengukuran Kadar Asam Humat Hasil Ekstraksi Kalium Hidroksida dengan Spektrofotometri Ultraviolet, Jurnal Kimia Khatuslitiwa. 2015; 5 (2):69-77.

Mohadi, R., Hidayati, N., Santosa, S.J. Narsito. 2008. Karakterisasi Asam Humat dari Gambut Indralaya, Ogan Ilir Sumatera Selatan, Jurnal Penelitian Sains. 11(1):411-420.

Schnitzer, M. 1986. Pengikatan Bahan Humat Oleh Koloid Mineral Tanah, dalam buku Interaksi Mineral Tanah dengan Organik Alami dan Mikroba, 1997, Huang, P. M dan Schnitzer, M. (editor), Gadjah Mada University Press, Yogakarta, 119-155.

DOI: http://dx.doi.org/10.26418/indonesian.v1i1.26041


  • There are currently no refbacks.

Creative Commons License

This work is licensed under a

Creative Commons Attribution 4.0 International License.